Abstract: Objective: To compare the effect of Maca extract in different process on human sperm function.Methods:Sperm suspension was prepared by the method of non- continuous density gradient in 20 infertile men,Maca ingredients were extracted by alkali soluble and acid sinking method,static and dynamic adsorption- desorption method,aqueous and hydroalcoholic extracting method.The effect of different Maca extract was analyzed on human sperm function by computer- aided sperm analysis system. Results: Values of PR( progressive),PR- A( progressive straight line) and PR- B( medium forward) in experimental group was separately higher 54.67%,52.74%,58.15% than control group( p < 0. 05). Sperm motility was the best when the Maca extract concertration was 0.1 g / m L,the value of STR( straightness,VSL / VAP) and LIN( linearity) in experimental group was separately higher 1.61% and 13.32% than control group( p < 0.05). Conclusion: Experiment showed that sperm motility could reach the peak,sperm quality was beterwhen Maca extract was at a suitable concentration.