Abstract: In order to find the appropriate artificial diet to replace the trash fish,effects of replacement of trash fish with formulated diets and mixed diets on free amino acids,tasty nucleotides and taste profiles in different parts of female Portunus trituberculatus were investigated in this study. The result indicated that the content of GMP,IMP and AMP in the gonads of mixed diets treatment and formulated diets treatment were reached the level of trash fish treatment,nevertheless,the content of AMP in the abdominal meat and the content of IMP in the hepatopancreas of formulated diets treatment was significantly lower than that of trash fish treatment(p < 0.05). In the mixed diets treatment,which decreased was the content of AMP in the hepatopancreas. There was no significant difference between the total amount of free amino acids in the gonad and abdominal meat of mixed diets treatment and trash fish treatment(p > 0.05),and significantly higher than that of the formulated diets treatment,furthermore,the total free amino acid content of the mixed diets treatment of the hepatopancreas was 1.44 and 1.21 times that of formulated diets treatment and trash fish treatment.Comprehensive analysis showed that the mixed diets treatment was more close to the trash fish treatment. As well as,the electronic tongue data showed that artificial feed were different slightly from trash fish treatment.