Abstract: In order to establish the rapid batch determination method,which could be used in the determination of the inulin in Taraxacum kok- saghyz Rodin root by enzyme mark instrument,3,5- dinitrosalicylic Acid Colorimetry( DNS) was used as the mainly methods. The results showed that the optimum conditions as follows,the wave length was 550 nm,the volume of color solution was 0.8 m L,the coloration time was 8 min.Under this condition,the calibration curve of this method had a liner range from 0 to 2.2 mg / m L with a regression equation of A = 0.6872C-0.0215( R~2= 0.9982),and the spotting recovery rate was from 96.67% to 102.33%.The method was sensitive,stable and reproducibility to operate,and it was suitable to be used for rapidly and quantitatively analysis of inulin in Taraxacum kok- saghyz Rodin.The results of the inulin in Taraxacum kok- saghyz Rodin,which had been detected by enzyme is more higher than by spectrophotometry.