蛹虫草子实体中类胡萝卜素的提取及抗氧化活性
Optimization of extraction process and antioxidant activity of carotenoids from fruiting body of Cordyceps Militaris
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摘要: 在比较蛹虫草子实体类胡萝卜素的溶剂提取法、超声波辅助提取法、微波辅助提取法、酸热提取法的基础上,对蛹虫草子实体中类胡萝卜素的提取工艺进行了优化,并分析了类胡萝卜素的体外抗氧化活性。实验结果表明:酸热提取法是几种常见提取方法中类胡萝卜素提取得率最高的一种方法;酸热法最优提取工艺为:盐酸浓度为1 mol/L,盐酸用量为20 m L/g,酸浸时间为30 min,热处理时间为5 min,蛹虫草中类胡萝卜素的得率达到1387μg/g。抗氧化实验结果显示:蛹虫草子实体中类胡萝卜素具有较好的体外抗氧化活性,在0~10 mg/m L的浓度范围内抗氧化能力随着类胡萝卜素浓度的提高而增加。4 mg/m L类胡萝卜素的DPPH自由基清除率达到96.57%,类胡萝卜素对铁离子螯合能力的EC50值为3.26 mg/m L,且还显示出了较好的还原能力。Abstract: Orthogonal experiment was used to optimize the extraction process of carotenoids from the fruiting body of Cordyceps Militaris based on the comparison results of solvent extraction method,ultrasonic assisted extraction method,microwave assisted extraction method and acid heat extraction method,and the antioxidant activity of carotenoids was also detected.The experiment results showed that the acid thermal method was better than other extraction methods,and the optimal extracting conditions of acid thermal method were: the concentration of hydrochloric acid of 1 mol / m L,the usage of hydrochloric acid of 20 m L per gram fruiting body,time of digestion of30 min,time of thermal treatment of 5 min.The yield of carotenoids reached to 1387μg / g.Anti- oxidation experiment results showed that carotenoids from fruiting body of Cordyceps Militaris had better antioxidant ability and the ability was increased with higher concentrations. At concentration of 4 mg / m L,the carotenoids could decrease96.57% of DPPH radicals and value of EC50 of the Chelating ability on iron ions was 3.26 mg / m L. In addition,the carotenoids of Cordyceps Militaris also revealed better reducing capacity.
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