Abstract: Collagens have been widely used in food,cosmetic and biomedicine for its unique physical and chemical properties and biological functions.However,collagens used in these fields were commonly extracted from animal tissues,thus its application was limited by the risk of animal- derived contaminating pathogens and difficulties in the preparation.Recombinant human collagen- like proteins have the some properties and functions as the natural collagens.In recent years,human collagen- like proteins have been expressed in a variety of expression systems.The new research progress of bio- production and purification of recombinant collagen- like proteins and their application have been reviewed in this paper. This work will be helpful to the large- scale preparation of the recombinant human collagen- like proteins in the future.