响应面法优化虎斑乌贼墨多糖提取工艺的研究

Study on condition optimizing of extraction technique of squid ink polysaccharide from Sepia pharaonis by response surface methodology

摘要: 为了探究虎斑乌贼墨多糖的最佳提取工艺,以虎斑乌贼墨为原料,通过单因素实验研究了加酶量、料液比、加热温度、加热时间和p H对虎斑乌贼墨多糖提取率的影响,在此基础上对加酶量、料液比、加热温度和p H进行响应面优化。实验结果表明:最佳组合为加酶量46 mg/g、料液比1∶19.7 g/m L、温度54.1℃、p H9.0、加热时间4 h,此时多糖得率高达2.140%。

Abstract: To explore the optimum extraction conditions of squid ink polysaccharides from Sepia pharaonis squid ink,this study was conducted to provide a theoretical basis for the development and utilization of squid ink. By single factor tests,enzyme dosage,solid- liquid ratio,reaction temperature,reaction duration and p H were investigated.And on the basis of single factor tests,according to the( Box- Behnken) experimental design protocol,enzyme dosage,solid- liquid- ratio,reaction temperature,and p H were selected to the four factors and the response surface experiment was carried out by response surface analysis. The results showed that their optimal conditions were defined as follows: enzyme amount 46 mg / g,solid- liquid- ratio 1∶ 19.7,reaction temperature 54.1 ℃and p H9.0. Under this condition,the polysaccharide yield was as high as 2.140%.