浒苔多糖的酶法降解及其工艺条件优化的研究
Study on enzymatic degradation for polysaccharide from Enteromorpha and its technical condition optimization
-
摘要: 采用生物酶法将浒苔多糖降解为分子质量较小、粘度较低的浒苔寡糖,以还原糖生成量作为指标,通过单因素和响应面法优化降解工艺,利用液相色谱和质谱技术对酶解产物进行了组分分析。结果表明:浒苔多糖降解酶能够实现对浒苔多糖的高效降解,最适酶解条件为加酶量1.5%、底物质量浓度14 mg/m L、初始p H=6.5、反应时间6 h、温度33℃,在此条件下,还原糖生成量为165.21 mg,降解率为61.21%,粘度由349.89 m Pa·s降低至2.05 m Pa·s。单糖组成结果表明酶解产物由鼠李糖、葡萄糖醛酸、葡萄糖、木糖组成,质谱分析表明酶解产物包括酸性寡糖片段和中性寡糖片段。Abstract: The purpose of this study was to hydrolyze PE for oligosaccharide using specific PE degradase. The enzymatic hydrolysis conditions were optimized. The saccharide composition of the hydrolysate fractions was analyzed by liquid chromatography and electrospray ionization mass spectrometry.The yield of reducing sugar was chosen as an evaluating indicator during single factor experiments and response surface methodology. Results showed that the enzyme could hydrolyze PE efficiently. The optimal hydrolysis condition was as follows: enzyme dosage 1.5%,substrate concentration 14 mg / m L,the initial p H6.5,reaction time 6 h,and hydrolysis temperature of33 ℃.The corresponding reducing sugar yield and hydrolysis efficiency was 165.21 mg and 61.21%,respectively.Besides,the viscosity of PE reduced from 349.89 m Pa·s to 2.05 m Pa·s.The HPLC results indicated that the monosaccharide composition of the hydrolysate was rhamnose,glucuronic acid,glucose and xylose.The results of mass analysis showed that degradase could degrade sulfate PE and neutral PE.
下载: