钙离子与pH诱导鲤鱼卵肽纳米颗粒的形成

黄海; 李八方; 曾名湧

doi:10.13386/j.issn1002-0306.2016.18.011

钙离子与pH诱导鲤鱼卵肽纳米颗粒的形成

Nanoparticles formation of carp eggs peptides induced by Ca2+ and pH values

摘要

摘要: 探讨钙离子和p H诱导鲤鱼卵肽（Carp egg peptide,CEP）形成纳米颗粒的条件及稳定纳米颗粒空间结构的作用力。通过浊度检测初步筛选CEP纳米颗粒形成条件,用粒径分析和电镜观察方法确认纳米颗粒的形成。通过次级键破坏实验分析稳定纳米颗粒空间结构的作用力,并通过红外光谱和圆二色谱分析CEP纳米颗粒形成后的空间结构变化。结果表明,当p H6.0⁹.0时,CEP能与一定浓度的钙离子结合形成粒径20¹00 nm的纳米颗粒,疏水作用和静电作用是稳定纳米颗粒的主要作用力,CEP形成纳米颗粒后伴随着β折叠的形成。CEP能够作为生物模板在温和条件下合成肽-钙纳米颗粒。

Abstract: To investigate the conditions of nanoparticles formation of carp egg peptide( CEP) induced by Ca²⁺and p H and to demonstrate the force of stabling their space structures. The conditions could be preliminary screened by turbidity testing and confirmed by particle size analysis and electron microscope observation. The forces of stabling nanoparticles could be determined through secondary bond destruction experiments and the changes of their space structures were studied using Fourier transform infrared spectroscopy( FTIR) and circular dichroism spectroscopy( CD).The results showed that nanoparticles whose diameters ranging from 20~100 nm were formed by CEP and Ca²⁺under p H6.0~9.0.The stabilities of the nanoparticles were mainly dependent on hydrophobic force and electrostatic force.The β- sheet formation was observed while CEP formed nanoparticles.CEP could be used as a biological template to synthesize peptide- calcium nanoparticles under mild conditions.