响应面法优化大鲵鱼油水酶法提取工艺

Optimization of extraction of Andrias davidianus oil by aqueous enzymes using response surface methodology

摘要: 通过单因素实验考察中性蛋白酶、木瓜蛋白酶、胃蛋白酶、胰蛋白酶和风味蛋白酶对大鲵油提取率的影响,确定木瓜蛋白酶为实验用酶;采用中心组合实验（CCD）对酶解工艺进行优化,确定酶解的最适工艺条件为:料液比为1∶1（w/w）,酶添加量为6800 U/g,p H5.8,酶解温度为50℃,酶解时间为130 min,在优化条件下大鲵油提取率为93.52%。对水酶法提取的大鲵油进行理化、感官分析,结果表明粗制大鲵油产品质量达到SC/T3502-2000二级粗鱼油指标要求。

Abstract: The effect of enzymes( neutral protease,papain,pepsin,trypsin and flavourzyme) on extraction rate of Andrias davidianus oil were studied by the single factor experiment,and papain was selected to be the enzyme for the experiment. Central composite design( CCD) were used to optimize the condition of aqueous enzymatic process. The optimal conditions were as follows: solid- to- liquid ration of 1 ∶ 1( w / w),papain added dosage of6800 U / g,p H5.8,hydrolysis temperature of 50 ℃,and hydrolysis time of 130 min,and under such condition the extraction rate was 93.52%.Chemical and sensory evaluation results showed that the crude Andrias davidianus oil extracted by aqueous enzymatic conformed to the secondary standards crude fish oil of SC / T 3502-2000.