响应面优化微波辅助法提取刺梨水不溶性膳食纤维工艺
Optimization of microwave-assisted extraction of insoluble dietary fiber (IDF) from Rosa roxburghii by response surface method
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摘要: 以刺梨为原材料,采用微波辅助法提取刺梨水不溶性膳食纤维。在单因素实验的基础上,采用DesignExpert V8.0.6软件设计响应面实验优化微波辅助法提取刺梨中水不溶性膳食纤维(IDF)的工艺。结果表明:影响微波辅助法提取IDF得率的主次因素为:提取温度>微波功率强度>液料比>微波时间。微波辅助提取刺梨IDF的最佳工艺参数为微波功率强度345 W/g,提取温度:63℃,微波时间:12 min,液料比:20 m L/g,此条件下刺梨IDF得率可达80.02%,与IDF得率理论值比较,其相对误差约为0.22%,且重复性好,验证了数学模型的准确可靠性。Abstract: Insoluble dietary fiber( IDF) was extracted from Rosa roxburghii by microwave- assisted extraction method.Based on the results of single factor experiments,the extraction procedure was optimized using response surface methodology( RSM) and Design- Expert V8.0.6 software.The order of influencing factors of IDF yield was:extraction temperature > the power of microwave > liquid- to- solid ratio > microwave- assisted extraction time. The optimum extraction conditions were as follows: the microwave power of 345 W/ g; extraction temperature of 63 ℃;microwave- assisted extraction time of 12 min; and liquid- to- solid ratio of 20 m L / g. Under the above optimum extraction conditions,the actual yield of IDF from Rosa roxburghii was 80.02%. The relative standard deviation between theoretical and practical yields was 0.22% and the results demonstrated a good reproducibility,indicating the effectiveness and reliability of RSM model.
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