响应面法优化微波辅助提取蛇六谷生物碱工艺

Optimization of microwave- assisted extraction for Konjac alkaloids by response surface methodology

摘要: 采用微波辅助提取法从蛇六谷中提取生物碱,并用酸性染料比色法进行定量分析,用红外光谱对提取的生物碱进行定性分析。在单因素(乙醇浓度、料液比、提取温度和时间)实验的基础上,选取料液比、微波提取温度和提取时间三个因素进行响应面分析,从而对蛇六谷生物碱提取工艺进行优化。结果表明,经优化后的蛇六谷生物碱最佳提取工艺为:以95%的乙醇为提取剂,料液比为1∶20.4(g/m L),在微波消解仪中提取时间为8 min,提取温度为60℃,此条件下蛇六谷生物碱得率为0.216%。

Abstract: The microwave- assisted extraction,infrared spectrum qualitative analysis and the acid dye colorimetry for quantitative analysis were carried out on the alkaloids from Konjac. Based on the single- factor( ethanol concentration,solid- liquid ratio,microwave time and temperature) tests,taken the solid- liquid ratio,extract temperature and extract time as independent variables and the content of the alkaloids from Konjac as dependent variable,the extract parameters of the alkaloids from Konjac were optimized by response surface methodology.The results showed that the optimized extract parameters were 95% ethanol as extraction solvent,solid- liquid ratio was1∶ 20.4( g / m L),extract time was 8 min,extract temperature was 60 ℃. Under the optimized extract conditions,the extraction rate of alkaloid reached 0.216%.