Abstract: To optimize the sterilizing parameters of chlorine dioxide for Salmonella spp.in oyster,the samples were inoculated with a cocktail of two Salmonella serotypes,S.Typhimurium( CICC22956) and S.Enteritidis( CICC21482).The single factor tests and response surface methodology( RSM) were applied.The impacts of different proportion,concentration of chlorine dioxide,sterilization time Salmonella spp. sterilization in oyster were investigated. Results showed that the optimal sterilizing conditions were as follows: the ratio of oyster to chlorine dioxide solution was1∶ 7 g / m L,the concentration of chlorine dioxide was 93 g / m L,and operating time was 30 min. The repeated test under these conditions showed that the reduced number of Salmonella pp. was 3.13 lg cfu / g. In conclusion,the optimal conditions for sterilization of fresh oysters could not only effectively prevent the contamination of Salmonella spp.,but also improve the quality and safety of fresh oysters.