表面增强拉曼光谱检测保健品中的盐酸吡咯列酮,盐酸罗格列酮与盐酸苯乙双胍
Surface- Enhanced Raman Spectroscopy( SERS) assay method for pioglitazone hydrochloride,rosiglitazone hydrochloride and phenformin hydrochloride in health care products
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摘要: 目的:建立一种用于检测盐酸吡咯列酮,盐酸罗格列酮与盐酸苯乙双胍的快速分析方法。方法:采用表面增强拉曼光谱(SERS)分析方法检测盐酸吡咯列酮,盐酸罗格列酮与盐酸苯乙双胍。结果:该方法检测盐酸吡咯列酮,盐酸罗格列酮与盐酸苯乙双胍,检测浓度分别为4、4、1μg/m L。该方法的检测结果受p H的影响,p H2.0时,上述三种降糖药的表面增强拉曼效应较显著。该方法可以用于检测保健品中添加的盐酸吡咯列酮,盐酸罗格列酮与盐酸苯乙双胍,检测浓度分别为200、200、100μg/m L。结论:该方法操作简便,所用时间短,前处理与检测时间一共只需15 min,适合现场快速筛查。Abstract: Objective: A fast assay method for pioglitazone hydrochloride,rosiglitazone hydrochloride and phenformin hydrochloride was developed.Method: A Surface- Enhanced Raman Spectroscopy( SERS) assay method was used to detect the pioglitazone hydrochloride,rosiglitazone hydrochloride and phenformin hydrochloride. Result: The concentration of the SERS method for pioglitazone hydrochloride and rosiglitazone hydrochloride was 4 μg / m L,and for phenformin hydrochloride was 1 μg / m L. The SERS spectrums under three different p H conditions were studied,and the results showed that the p H of the system had obvious effect on the SERS,and the SERS was most enhanced when the p H was 2.0. For detecting health care products,the concentration of the SERS method for pioglitazone hydrochloride and rosiglitazone hydrochloride was 200 μg / m L,and for phenformin hydrochloride was100 μg / m L.The SERS method was simple and rapid and could be finished in fifteen minutes.Conclusion: The SERS method could be a convenient tool for field detection of health care products.
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