乳杆菌代谢所产生的抑酵母活性物质的分离纯化

Study on separation of antiyeast active substances produced by Lactobacillus

摘要: 从内蒙古传统乳酸发酵食品中筛选出具有抑酵母作用的乳杆菌,对其代谢产生的抑菌物质进行分离纯化。结果表明:ALAC-3和ALAC-4菌株的代谢产物粗提液通过Sephadex G-100凝胶过滤层析分离纯化后,分别得到抑菌活性组分G100-3和G100-5,经过SDS聚丙烯酰胺凝胶电泳检测均达到电泳纯,相对分子质量分别为80.49 ku和40.32 ku。经过薄层色谱法证明G100-3和G100-5为单一成分的抑菌物质。

Abstract: The characteristics of antiyeast substances produced by Lactobacillus,which were isolated from traditional dairy fermentation food in Inner Mongolia were studied. The results were showed as follows: The active substances of ALAC- 3 and ALAC- 4 were separated and purified by G- 100 Sephadex gel filtration chromatography.And then the G100-3 and G100-5 were obtained respectively.They were analytically pure and the molecular weights were about 80.49 ku and 40.32 ku respectively through SDS- PAGE. G100- 3 and G100- 5 were judged separately as the single substances according to the thin- layer chromatography.