Abstract: To extend the shelf life of thousand layer cake,the preservative formula optimization and the shelf-life prediction had been developed. Through single and compound preservative test,the results showed that the inhibitory effect of sodium dehydroacetate on mold of cake was better than the effect of calcium propionate,and the more sodium dehydroacetate was used,the better the effect was. Sodium dehydroacetate and calcium propionate had synergistic effect,and the best formula was sodium dehydroacetate 0.375 g/kg mixed with calcium propionate 0.625 g/kg.The predicted shelf life of thousand layer cake,under the condition of 20 ℃ and humidity60%,was 98 d to 117 d,using Svante Arrhenius model to forecast the shelf life.There was a margin of error of-23.9% ~-10.1% some deviation between ASLT group and the normal control group,which shelf life was 89 d.However,for the generally speaking,the research not only improved the shelf-life of traditional cake,but also rapidly and effectively predicted the shelf life of thousand layer cake. All of these could shorten the cycle of products from R & D to the market,and then save the cost of manufacture,and it was worth popularizing.