Abstract: Acetylated cassava starch,corn starch and carrageenan were used to prepare complex film. Based on the characteristics including viscosity,solubility and inflation rate of cassava modified starch,corn starch and carrageenan,the three kinds of composite films with proportion of 5 ∶ 3 ∶ 2,2 ∶ 6 ∶ 2 and 1 ∶ 7 ∶ 2( acetylated cassava starch: corn starch: carrageenan) were made and tested on the light transmittance of membrane,water vapor transmittance and moisture absorption rate. The results revealed that No.1 film containing high concentration of acetylated cassava starch had the maximum tensile strength and elongation which were 5.13 MPa and 6.0%,respectively. Acetylated cassava starch had a significant impact on water vapor transmittance and moisture absorption rate of membrane when the content of carrageenan was constant.The moisture absorption,water vapor transmittance and the light transmittance of membrane of No.1 film was 40.16%,0.49 g·mm/m²·h·k Pa and 44.5%( 450 nm),while the characteristics of No.3 film was 21.39%,0.34 g·mm/m²·h·k Pa and 34.80%( 450 nm),respectively. Overall,acetylated cassava starch and carrageenan were the key compositions for the film preparation.