不同处理方法对豆渣水溶性膳食纤维结构与功能的影响
Effects of treatment methods on structural and characteristics of soluble dietary fiber extracted from soybean dregs
-
摘要: 采用不同处理方法制备得到四种豆渣水溶性膳食纤维,分别为原豆渣直接提取的水溶性膳食纤维SDF1,纤维素酶酶解豆渣提取的SDF2,挤压豆渣提取的SDF3和挤压-酶解复合处理豆渣提取的SDF4。通过粒径分析仪、电子显微镜、分光光度仪、黏度计和体外抗氧化试剂盒等研究对比不同的SDF的结构和功能特性。研究结果表明,SDF4粒度最小,体积平均粒径为（39.311±0.657）μm;SDF2和SDF4颗粒表面结构更加疏松,棱角消失;SDF4黏度最低,阳离子交换能力和胆固醇吸附能力最高,体外抗氧化能力最大,其铁还原力为（0.169±0.004）mmol/g,ABTS+·清除能力为（0.806±0.028）mmol/g,·OH清除能力为（305.739±4.965）U/m L。挤压、酶解处理对水溶性膳食纤维结构和功能性有显著影响（p<0.05）。Abstract: Four kinds of soluble dietary fibers( raw soybean dregs SDF1,enzymatic hydrolysis SDF2,extruded SDF3,extrusion-enzymatic SDF4) were extracted from soybean dregs and their structural and functional properties were investigated using electron microscope,laser particle size analyzer,ultraviolet spectrophotometer,rotary viscomete and in vitro antioxidant Kits. The results showed that the volume average particle size of SDF4 was the smallest of( 39.311 ± 0.657) μm.The surface structures of SDF2 and SDF4 particles were much looser with almost no edges.SDF4 exhibited the lowest viscosity,stronger cation-exchange and cholesterol-adsorption capacity. The FRAP,the inhibition capacity on ABTS+·and·OH radical of SDF4 was the largest of( 0.169 ± 0.004) mmol/g,( 0.806± 0.028) mmol/g and( 305.739 ± 4.965) U/m L,respectively.Extrusion-enzymatic hydrolysis had a significant impact on the structure and function of soluble dietary fiber( p < 0.05).
下载: