阿魏侧耳胞外多糖对小鼠巨噬细胞的激活作用

王晶; 张春丹; 杨利敏; 解春艳; 杜娟; 闫训友; 吴智艳

doi:10.13386/j.issn1002-0306.2016.10.065

阿魏侧耳胞外多糖对小鼠巨噬细胞的激活作用

Activation effects of extracellular polysaccharides produced by Pleurotus ferulae Lanzi on murine macrophage

摘要

摘要: 利用巨噬细胞体外培养体系,研究阿魏侧耳胞外多糖对小鼠巨噬细胞的激活作用。无菌获得小鼠腹腔巨噬细胞,中性红吞噬实验检测细胞吞噬活性;荧光探针标记和Griess反应分别检测细胞内外NO的含量,酶联免疫吸附法检测细胞内一氧化氮合成酶（NOS）、培养上清中肿瘤坏死因子-α（TNF-α）、白细胞介素-1（IL-1）和白细胞介素12（IL-12）的含量。结果表明,与空白组相比,25²00 mg/L阿魏侧耳胞外多糖可显著增强巨噬细胞的吞噬活性（p<0.05）,增加细胞因子IL-1（p<0.05）和IL-12（p<0.05）的分泌量;50²00 mg/L阿魏侧耳胞外多糖可显著提高细胞NOS（p<0.05）和NO（p<0.05）的含量以及TNF-α的分泌量（p<0.05）。因此,一定浓度阿魏侧耳胞外多糖对体外培养的小鼠巨噬细胞具有激活作用。

Abstract: The culture system of macrophages was established to investigate the activation effects of extracellular polysaccharides produced by Pleurotus ferulae Lanzi( IPP) on murine macrophage. Murine macrophages were cultured in sterile environment. Phagocytic activity,the content of nitric oxide synthetase( NOS) and nitric oxide( NO),the levels of tumor necrosis factor-α( TNF-α),interleukin-1( IL-1),and interleukin-12( IL-12) in culture supernatant were examined with neutral red phagocytosis test,fluorescein labeled probe technique,griess reaction and enzyme- linked immunosorbent assay( ELISA),respectively. The results showed that IPP in the range of 25 ~200 mg / L reduced absorbance of neutral red in cytoplasm( p < 0.05),improved the secretion level of IL- 1( p <0.05) and IL- 12( p < 0.05) in culture supernatant. And IPP in the range of 50 ~ 200 mg / L increased the content of NOS( p < 0.05) and NO( p < 0.05),and the secretion level TNF-α( p < 0.05). So murine macrophages cultured in vitro could be activated by IPP at a certain concentration.