甘草不同溶剂提取物对AChE、ACE以及α-葡萄糖苷酶的抑制活性和对亚硝酸根清除活性的研究

赵岩; 彭蕾; 郜玉钢; 何忠梅; 杨鹤; 刘双利; 张连学

doi:10.13386/j.issn1002-0306.2016.10.029

甘草不同溶剂提取物对AChE、ACE以及α-葡萄糖苷酶的抑制活性和对亚硝酸根清除活性的研究

Study on ACh E,ACE,α- glycosidase inhibitory activity and nitrite scavenging activity of the licorice extracts

摘要

摘要: 目的:研究甘草不同溶剂提取物对α-葡萄糖苷酶、乙酰胆碱酯酶（ACh E）、血管紧张素转化酶（ACE）活性的影响以及对亚硝酸根离子的清除能力。方法:采用高效液相色谱法测定甘草不同溶剂提取物对ACE的抑制作用,采用分光光度法测定其对α-葡萄糖苷酶、ACh E的抑制作用及对亚硝酸根离子的清除能力。结果表明:甘草石油醚、乙酸乙酯、乙醇提取物均对α-葡萄糖苷酶有较好的抑制作用,其IC₅₀值分别为3.212、0.151、0.891 mg/m L,优于阳性药阿卡波糖;甘草提取物乙酸乙酯部位、水部位对ACh E有较强的抑制作用,其IC₅₀值为0.489、0.872 mg/m L,优于阳性药利斯的明;甘草乙酸乙酯提取物对亚硝酸根离子有较强的清除能力,其IC₅₀值为1.068 mg/m L,优于阳性药V_C;甘草乙酸乙酯提取物对ACE有较强的抑制作用,其IC₅₀值为0.815 mg/m L,优于其阳性药卡托普利。在这5种甘草不同溶剂提取物中,甘草乙酸乙酯提取物抑制活性最强。结论:甘草乙酸乙酯提取物具有很强的α-葡萄糖苷酶、ACh E、ACE抑制作用且对亚硝酸离子有很好的清除能力。

Abstract: Objective: To research the effect of different layer of licorice extracts on α- glycosidase enzymes,Ach E and ACE activities and the influence of the effect of nitrite scavenging activity. Methods: Five kinds of licorice extracts were extracted from Glycyrrhiza uraleusis Fisch.Their ACE inhibitory activity was determined by HPLC.The extracts' nitrite scavenging capability and their α- glucosidase and ACh E inhibitory activity were measured by spectrophotometry,respectively. Results: The evaluation results showed that petroleum ether,ethyl acetate and ethanol fractions of licorice possessed α- glucosidase inhibitory effect and their IC_(50) value was 3.212,0.151,0.891 mg / m L,which were more potent than that of acarbose,ethyl acetate,water fractions of licorice possessed ACh E inhibitory effect and their IC_(50) value was 0.489,0.872 mg / m L,which were more potent than that of rivastigmine.Ethyl acetate fractions of licorice possessed nitrite scavenging activity and their IC_(50) value was 1.068 mg / m L,which was more potent than that of VC.Ethyl acetate fractions of licorice possessed ACE inhibitory effect and their IC_(50) value was 0.815 mg / m L,which was more potent than that of captopril,so ethyl acetate fractions of licorice was the most potent inhibitory in five kinds of licorice extracts. Conclusion: Ethyl acetate fractions of Glycyrrhiza uraleusis Fisch have shown high ACE,ACh E and α- glycosidase inhibitory capacity and nitrite scavenging activity so they will be further guided isolation and purified to obtain active compounds.