Abstract: The antibacterial activities of abalone shell calcinate against the common pathogenic bacteria were studied.Both of filter paper and Oxford cup double agar diffusion methods were used to detect the constrain on the specific bacteria of different temperature rangesbalone shell calcinate,while the minimal inhibitory concentration( MIC) was determined by agar gradient dilution method.The results showed that abalone shell calcinate inhibited strongly against those common pathogenic bacteria in the calcining temperature ranges ≥ 800 ℃,especially significantly for Staphylococcus aureus and Pseudomonas aeruginosa( p < 0.05). MIC was 1 mg / m L and0.5 mg / m L. And within a certain temperature range,the inhibition ability increased with the calcining temperatures,with the optimal antimicrobial calcining temperature at 1100 ℃.