酸降解法制备褐藻寡糖抗氧化性的研究

胡博旸; 聂莹; 孙路; 韭泽悟; 殷丽君; 周辉; 李茉莉; 程永强

doi:10.13386/j.issn1002-0306.2016.10.018

酸降解法制备褐藻寡糖抗氧化性的研究

Antioxidant evaluation of alginate oligosaccharides prepared by acid hydrolysis with different conditions

摘要

摘要: 从褐藻胶中提取出聚甘露糖醛酸和聚古罗糖醛酸,利用盐酸降解聚甘露糖醛酸和聚古罗糖醛酸1、2、6 h分别制备了甘露糖醛酸组分（M₁、M₂和M₃）以及古罗糖醛酸组分（G₁、G₂和G₃）,并且以肌肽和甘露糖为对照评估寡糖对DPPH自由基、超氧自由基和羟自由基的清除作用。结果表明,甘露糖醛酸以及古罗糖醛酸对DPPH自由基、超氧自由基具有良好的清除作用,且清除作用随着寡糖中还原糖含量的增加而增加。在DPPH体系中,M₃的清除效果要好于肌肽,G₃的清除效果与肌肽相近。在超氧自由基体系中,M₃的清除作用高于M₂和M₁,而G₃的清除作用略低于肌肽。在羟自由基体系中,甘露糖醛酸和古罗糖醛酸的清除效果低于肌肽和甘露醇。实验表明,酸法制备的褐藻寡糖具有较强的抗氧化能力,且抗氧化效果与寡糖的平均聚合度含量有关。

Abstract: Polymannuronates and polyguluronates were prepared from alginate,and subsequently treated with hydochloric acid for 1 h,2 h and 6 h to obtain mannuronic acid oligomers( M-1、M_2 and M_3) and guluronic acid oligomers( G_1、G_2 and G_3). Besides,carnosine and mannose were used as control to evaluate the scavenging effect of oligosaccharide on the DPPH,superoxident radical and hydroxyl radical. The results showed that Polymannuronates and Polyguluronates had strong scavenging effects on DPPH and superoxident radical,and the scavenging effect became more efficient with the increasing concentration of oligosaccharide. M_3 had stronger effects than carnosine on scavenging DPPH.G_3 was similar with carnosine referring to scavenging effect.M_3 had a greater effect than M_2 and M_1 on scavenging superoxident radical,while G_3 was slightly lower than carnosine.Polymannuronates and Polygluronates showed less effect on scavenging hydroxyl radical than carnosine and mannite.According to the data,the average degree of polymerization of alginate oligosaccharides had a greater effect on antioxidant ability.