不同分子量黑籽瓜种子多肽抗氧化能力的研究
Study on the in vitro antioxidant capacity of different molecular weight polypeptides of black seed- watermelon seeds
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摘要: 目的:研究不同分子量黑籽瓜种子多肽的抗氧化能力的差异。方法:利用超滤技术对黑籽瓜种子蛋白酶解产物（SWSPs）进行多级分离,得到不同分子量分布的五种组分（SWSPs-L、SWSPs-1、SWSPs-3、SWSPs-5、SWSPs-10）,然后以七种抗氧化能力评价体系分别对五种组分进行评价。结果:不同分子量黑籽瓜种子多肽的抗氧化能力不尽相同,分子量在13 ku的组分SWSPs-1对超氧阴离子自由基的清除能力最强,而分子量<1 ku的组分SWSPs-L则表现出了多重抗氧化机制,该组分与等浓度的EDTA具有相近的螯合效果。结论:黑籽瓜种子多肽的抗氧化能力与其分子量分布和氨基酸组成密切相关,是各方面综合作用的结果。Abstract: Objective: To study the differences between antioxidant abilities of polypeptides with different molecular weights extracted from black seed- watermelon seeds.Methods: Multi- stage separation and ultrafiltration techniques were used to treat the enzymatic hydrolysates( SWSPs) of black seed- watermelon seeds protein,and five constituents( SWSPs- L,SWSPs-1,SWSPs-3,SWSPs-5,SWSPs-10) were separated and collected.Then the antioxidant abilities of these constituents were investigated through seven evaluation systems.Results: The antioxidant abilities were obviously different in these five constituents separated from black seed- watermelon seeds.SWSPs-1 with 1~3 ku molecular weights showed the highest superoxide anion radical- scavenging activity while SWSPs- L with molecular weights lower than 1 ku showed multiple antioxidant capacities and it had the similar metal ion chelating activity with EDTA under the same concentration.Conclusions: The antioxidant abilities of polypeptides extracted from black seed- watermelon seeds were closely related to their molecular weight distributions and amino acid compositions,and it was the result of comprehensive effect of all aspects.
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