桑叶总黄酮对2型糖尿病模型大鼠肝脏的保护作用

谭林; 刘冬恋; 杨春梅; 杨霞; 游均梅; 牟倩云

doi:10.13386/j.issn1002-0306.2016.08.063

桑叶总黄酮对2型糖尿病模型大鼠肝脏的保护作用

Effect of total flavonoids of mulberry leaves on the liver and mechanisms in type 2 diabetic rats

摘要

摘要: 目的:探讨桑叶总黄酮对2型糖尿病（T2DM）大鼠肝脏的保护作用。方法:选用雄性wistar大鼠,高脂饲料喂养且用链脲佐菌素诱导,复制T2DM模型,模型成功后随即分成模型组、二甲双胍组、桑叶总黄酮高、中、低(200、100、50 mg·kg^-1·bw^-1)剂量组,各药干预4周后,比较各组大鼠的体重和空腹血糖值;称重肝脏,计算肝脏指数;测定血清中甘油三酯（TG）、总胆固醇（TC）和丙氨酸氨基转移酶（ALT）水平;测定肝脏中肝糖原水平,葡萄糖激酶（GK）活性;对肝脏进行HE染色,做病理组织学检查。结果:与模型组相比,桑叶总黄酮高剂量组能降低T2DM大鼠的体重,为（390.79±77.27）g;降低TC、TG和ALT水平,分别为（2.25±0.58）mmol·L^-1、（0.92±0.36）mmol·L^-1和（69.36±5.58）U·L^-1;增加肝糖原含量,为（12.22±1.50）mg·g^-1;提高GK活性,为（10.33±1.30）m IU·min^-1·mg protein^-1;减轻肝脏脂肪变性,改善肝脏病理。结论:桑叶总黄酮在预防和改善T2DM肝损伤方面具有一定的应用开发潜力。

Abstract: Objective:To observe the effect of total flavonoids of mulberry leaves on liver in type 2 diabetic rats.Methods:The male wistar rats were fed with high fat diet and streptozotocin to induce the type 2 diabetic model and the model rats were divided into five groups randomly:model group,metformin group,total flavonoids of mulberry leaves dose groups(200,100,50 mg·kg^-1·bw^-1i.g). After 4 weeks,levels of weight,blood glucose,organ coefficient,total cholesterol(TC),triglyceride(TG) and alanine aminotransferase(ALT) in serum were observed;hepatic glycogen,hepatic GK activity,and liver samples were observed pathologically with HE staining. Results:Compared with the model group,total flavonoids of mulberry leaves high dose group could reduce the levels of weight(390.79±77.27) g,TC(2.25±0.58) mmol·L^-1,TG(0.92±0.36) mmol·L^-1 and ALT(69.36±5.58) U·L^-1,increase the content of hepatic glycogen(12.22±1.50) mg·g^-1,improve the activity of GK( 10. 33 ± 1. 30) m IU·min^-1·mg protein^-1, obviously alleviate the histopathologic changes including fatty degeneration. Conclusion:It showed that total flavonoids of mulberry leaves had potential to be developed in the aspect of preventing and improving liver damage in type 2 diabetic.