Abstract: In order to analyze the influence of resveratrol(Res) on immunoregulatory activity of mouse splenic lymphocyte. Aseptic isolation of mouse spleen to prepare lymphocytes which were cultured in RPMI 1640 supplemented with 10 % fetal calf serum. The culture media was added Concanavalin A( Con A),lipopolysaccharide(LPS) as well as Res with different concentration,including 5,10,20,40 μg/m L. After being cultured for different time,MTT method was adopted to detect the proliferation of lymphocytes. ELISA method was used to detect the level of lymphocytes secrete IL-4 and IL-12. The flow cytometry was used to detect the cell cycle and cytomembrane surface markers of lymphocytes. Res could promote the proliferation of mouse splenic lymphocyte. The mixture of Res and Con A could promote the proliferation of mouse splenic lymphocyte together, but the mixture of Res and LPS could not promote the proliferation of mouse splenic lymphocyte together. Res could increase the secretion of IL-4 and IL-12 and enhance the ratio of CD4+/CD8+of mouse splenic lymphocyte as well as improve the percentage that the cells in cycle of splenic lymphocyte enter of the cells in S phase. Res could enhance the immunoregulatory activity of mouse splenic lymphocyte.