Abstract: HPLC was applied to separate and determine polyphenols in cherry.The conditions of pretreatment,solid- phase extraction and chromatography were investigated. The optimal pretreatment and chromatographic separating conditions were established : extraction solvent was 60% methanol( 2% formic acid),the column leacheate was 10% methanol( 0.1% formic acid),column eluent was acetonitrile( 5% formic acid). Under this condition,the recovery of the polyphenols was higher than 70%. Meanwhile,mobile phase was water( 2% formic acid) and acetonitrile,column temperature was 40 ℃. Through comparison with standar sample,vanillic acid,catechin,caffeic acid,ruitin,p- coumaric acid was detected in cherry. The content of the polyphenols was in the range of 0.365 ~ 240.617( mg / kg DW). This method was rapid,sensitive,simple,accurate and less impurity interference,and rhe polyphenols peaks were separated well.