响应面优化超声提取杨桃多酚的工艺研究
Optimization of ultrasonic- assisted extraction of polyphenol from Carambola by response surface methodology
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摘要: 利用响应面法对杨桃总多酚的超声提取工艺进行优化。以杨桃为原料,总多酚提取得率为评价指标,在单因素实验的基础上,选取超声时间、乙醇浓度、液料比、超声温度进行了四因素三水平的Box-Behnken中心组合研究,并运用Design Expert 8.05b软件对实验数据进行分析。结果表明,最佳超声提取工艺条件为:超声时间为31 min,乙醇浓度为59%,液料比为53∶1 m L/g,超声温度为60℃,此时杨桃总多酚提取得率为28.5 mg/g。与响应面预测值相比,验证实验结果吻合性良好。Abstract: The response surface methodology was used to optimize ultrasound- assisted extraction of total polyphenols from Carambola.With Carambola as raw material,the extraction rate of total polyphenols for evaluation index,then based on single factor experiment,an ultrasonic procedure for the extraction of total polyphenols from carambola was established.A three- level- four- factor test was employed in this study to optimize the factorial parameters( ultrasonic time,ethanol concentration,ratio of solvents to raw material and ultrasonic temperature) by employing Box- Behnken design of response surface methodology and Design Expert 8.05 b software was used to analyze test data.The result showed that the best ultrasonic extraction technology conditions were as follows:ultrasonic time 31 min,alcohol concentration 59%,ratio of solvents to raw material 53 ∶ 1 m L / g,ultrasonic temperature 60 ℃,the carambola total polyphenols obtained rate was 28.5 mg / g.The experimental vaule was basically agreed with the predictive value of response surface analysis.
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