Abstract: In order to optimize the high pressure carbon dioxide( HPCD) treatment on button mushroom( Agaricus bisporus) preservation,the effects of treatment pressure,time and temperature on the firmness and color of button mushroom were studied.Moreover,on the basis of single- factor test,response surface methodology was used to optimize the conditions of button mushroom preservation. The results showed that the optimum HPCD treatment conditions of treatment pressure,time and temperature were 0.3 MPa,3 min and 17 ℃,respectively. Under these conditions,the firmness and color of button mushroom can be maintained better than control. After storage 8 d at low temperature,the firmness and BI of button mushroom were 191.7 N( increased by 18.85%) and 33.13( decreased by 16.80%),respectively.Thus,HPCD technology can be applied to button mushroom preservation which has a good prospect.