响应面法优化杨树花多糖超声提取工艺
Optimization of ultrasound- assisted extraction( UAE) of polysaccharides from Flos Populi with response surface methodology
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摘要: 采用Plackett-Burman设计结合Box-behnken响应面法优化杨树花多糖的超声提取工艺。以杨树花多糖得率为考察指标,对影响杨树花得率的超声功率、超声频率、提取温度、提取时间、提取次数、液料比、颗粒大小、醇沉时间、醇沉浓度等9个因素,采用Plackett-Burman设计从中筛选出具有显著效应的因素-超声功率、提取温度、提取时间;进一步以杨树花多糖得率为考察指标,采用Box-behnken设计响应面法对以上三个因素进行考察,确定最佳工艺条件为:超声功率195 W,提取温度66℃,提取时间43 min,以此条件提取的多糖含量为63.18±1.57 mg/g,与理论预测值仅相差2%3%。Abstract: To investigate and optimize the ultrasound- assisted extraction procedure for the water- soluble Polysaccharide from the Flos Populi,important variables and their levels were obtained using Plackett- Burman design and Box- Behnken design.A mathematical model was developed to show the effects of each variable and their combinatorial interactions on extraction yield of Polysaccharide. A high coefficient of determination( R2=98.77%) indicated good agreement between the experimental and predicted values of Polysaccharide yield. The optimum levels of these significant parameters were determined using response surface methodology( RSM),which revealed these as follows: ultrasonic output power 195 W,extraction temperature 66 ℃,extraction time43 min. Under these conditions,63.18 ± 1.57 mg / g of polysaccharide was extracted,which had a difference of2% ~3% with the predicted value.
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