Abstract: In order to promote the growth of Lactobacillus casei,a new cultivation method was developed by using plant resources.Making use of response surface methodology based on single factor optimization experiment,it is the first time to optimize the effect of WIC natural biologics primarily,which could alleviate the environmental pollution.The results proved that,WIC natural biologics could positively promote growth of Lactobacillus casei.With the applying of response surface analysis techniques,the optimal promotional growth conditions could be achieved as: the temperature of medium was 39.51 ℃,the initial p H of medium was 6.16,the inoculation quantity was 1.34%,the optimum concentration of WIC biologics preparation training was 12.56 mg / m L,the actual OD_610nm value of Lactobacillus was 2.227,and colonies total number reached 7.3 × 10~9 CFU / m L at last. This thesis would provide a theoretical basis for the application of natural biologics.