Abstract: The spoilage molds which caused the corruption of fresh- cut apple were identified,and the rot ability of molds were compared in vivo. 13 Strains of molds were isolated from fresh- cut apples,in which there were 7Penicillium strains,1 Aspergillus strain,3 Cladosporium strains,1 Acremonium strain,and 1 Alternaria strain by morphological character methtods.The different penicillium strains were identified by the cultivation characters and microscopic examination in different medium including CA,CYA,G25 N. The results showed that 5 types of Penicillium were identified, which were P.crustosum, P.expansum, P.miczynskii, P.aurantiogriseum and P.viridicatum.The in vivo experiments were used to determine type of molds spoilage or not. Five spoilage molds were identified,which were P.viridicatum,P.expansum,P.crustosum,A.alternata,and A.niger. And five kinds of molds could not rot apple were indentified as P.aurantiogriseum, A.terricola, C.sphaerospermum,C.cladosporioides,and P.miczynskii. The order of rot ability of 5 spolilage molds was P.crustosum,P.expansum,P.viridicatum,A.alternata,A.niger.