植物乳杆菌LB-17产γ-氨基丁酸培养基优化

Optimization of fermentation conditions for the production of γ-aminobutyric acid by Lactobacillus plantarum

摘要: 以植物乳杆菌（Lactobacillus plantarum Lb-17）为发酵菌株,以发酵产物γ-氨基丁酸产量为检测参数,对植物乳杆菌发酵产γ-氨基丁酸的发酵培养基进行优化。利用单因素实验和Box-Behnken响应曲面实验对发酵培养基进行优化得到最优培养基为:葡萄糖12.0 g/L、酵母粉18.0 g/L、Ca²⁺ 55.0 mmol/L、Mg²⁺ 60.0 mmol/L、L-谷氨酸钠26.0 g/L。优化后,植物乳杆菌Lb-17发酵γ-氨基丁酸产量达8.037 g/L,是优化前5.49 g/L提高1.5倍。

Abstract: Lactobacillus plantarum Lb-17 was fermented to produce γ- aminobutyric acid,using the production of GABA as a parameter.And then single factor test andresponse surface analysis were used to optimize the culture medium. The optimum medium constitution was as follows: glucose 12.0 g / L,yeast powder 18.0 g / L,Ca²⁺ 55.0 mmol / L,Mg²⁺ 60.0 mmol / L,L- Sodium glutamate 26.0 g / L. The yield of γ- aminobutyric acid was 8.037 g / L after cultivated in optimized medium,which was 1.5 times that of the yield before.