反相微乳法与碱法、酶法制备的茶蛋白功能性质比较研究
Comparison of functional properties of tea protein in reverse micelles,alkali and enzymatic
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摘要: 本文以绿茶茶渣为原料,采用新兴的反相微乳法提取制备茶渣蛋白,以开发利用茶渣蛋白为目标,对茶渣蛋白的功能特性展开研究,并与碱溶酸沉法、酶法茶蛋白功能性质比较分析。对CTAB-Tween80反相微乳提取制备的茶渣蛋白的功能性质进行研究,结果表明:茶渣蛋白在p H4.0时溶解度最低,溶解度随p H的增加呈现先降低后升高的趋势,其吸油性为2.54 g/g,持水力为1.78 g/g,乳化性为60.27%、乳化稳定性为84.56%,发泡性为68.69%、泡沫稳定性为59.30%。表明反相微乳法制备的茶渣蛋白除了在吸油性、持水力及泡沫稳定性方面与碱溶酸沉法和酶法稍有差异外,乳化性、乳化稳定性及发泡性等均优于碱溶酸沉法和酶法,因而反相微乳法可较好的保持茶渣蛋白的功能特性。Abstract: To develop the utilization of tea residue protein,the effects of reverse micelle system CTAB- Tween80 on some functional properties of tea residue protein were investigated,compared with the effects of alkali- solution and acid- isolation as well as enzymatic method. The results showed that tea residue protein had the lowest solubility at p H4.0,then solubility had a rising trend with increasing p H. The oil absorption capacity and the water absorption capacity were 2.54 g / g and 1.78 g / g,respectively,while the foam stability was 59.30%. Besides,the emulsibility was 60.27% with reverse micelle system CTAB- Tween80 preparation,the number was 84.56% in terms of emulsion stability.And the foam capacity valued 68.69%.Tea residue protein with reverse micelle system CTABTween80 preparation can form gel. In summary,tea residue protein with reverse micelle system CTAB- Tween80 preparation showed excellent emulsibility,emulsion stability and foam capacity despite its slightly poorer performance in the oil absorption capacity,water holding capacity and foam stability test,in comparison with tea residue protein with either alkali- solution and acid- isolation or enzymatic method,so reverse micelle system can keep the tea residue protein functional properties better.