海洋骨胶原低聚肽钙的分离纯化及结构鉴定

Separation,purification and structural identification of calcium- chelating Marine bone collagen oligopeptides

摘要: 以三文鱼骨作为原料,以碳酸钙作为钙源制备出海洋骨胶原低聚肽钙,对其总蛋白含量、螯合率、得率进行测定,结果表明总蛋白含量为62.56%±0.26%,螯合率为51.38%±0.09%,螯合物得率为42.06%±0.10%。然后利用反相高效液相色谱（RP-HPLC）对其进行分离纯化,收集得到8个组分峰,利用Q-TOF质谱仪测定肽序列,共分析出22个肽段,分子量大多在1000 u以下。

Abstract: Calcium- chelating Marine bone collagen oligopeptides( MBCP- Ca) were prepared by using salmon bone as raw material and calcium carbonate as calcium resource.The total protein content,chelating rate and yield were determined. Results showed that the total protein content,chelating rate and yield were 62.56% ± 0.26%,51.38% ± 0.09% and 42.06% ± 0.10%,respectively.Then,MBCP- Ca was separated and purified by reversed phase high performance liquid chromatography( RP- HPLC).A total of 8 fractions were collected and subjected to Q- TOF mass spectrometer to identify peptide sequences. A total of 22 peptide sequences were identified,and most molecular weights of the peptides were below 1000 u.