Abstract: Thermophilic Bacillus was the main contaminating bacteria during the process of dairy products. It affects quality and safety of the product. The formation of biofilm was the main reason for Thermophilic Bacillus to contaminate dairy products. Formation features,hazards and precautionary measures of Thermophilic Bacillus biofilm were reviewed in this paper,providing a future reference for the research of dairy production and its related fields.