Abstract: Objective : To study the extraction process of Perilla frutescens( L.) Britt. leaves flavonoids and purification process. Methods:The extraction conditions of flavonoids of Perilla frutescens(L.) Britt. leaves were optimized by response surface design and single factor experiment. The flavonoids from Perilla frutescens(L.)Britt. leaves were purified by AB-8 macroporous resin and preparative chromatography. Results:The optimal extraction conditions were:ethanol concentration,ratio of liquid to material,extraction temperature,initial microwave power, and extraction time were 63 %, 41 ∶ 1(m L / g), 72 ℃, 500 W and 7 min, respectively. Under these conditions,the yield of flavonoids was 7.81%. The optimal purification conditions of macroporous resin were:the sample solution passed through the resin column at a flow rate of 2 BV/h and p H2. Subsequently,the column was eluted by 70% alcohol at a flow rate of 3 BV/h. The purity of flavonoids increased from 21.24% to 53.10%.The flavonoids were divided into two groups by preparative chromatography. Conclusion:The optimum extraction technology of flavonoids from Perilla frutescens( L.) Britt. leaves by response surface was convenient and effective. The results indicated that the feasibility of macroporous resin and preparative chromatography for purification of flavonoids.