中心组合设计优化合生元酸马乳中乳酸菌的生长

Optimization of growth of lactic acid bacteria in synbiotics koumiss by central composite design

摘要: 为了研究四种不同低聚糖对合生元酸马乳中乳酸菌生长的影响,通过单因素实验筛选出促进酸马乳中乳酸菌生长的3种低聚糖,并运用中心组合实验及响应面分析,优化了3种低聚糖的最佳添加量。结果表明:3种低聚糖的最佳添加量为:低聚半乳糖1.30%(W/V)、低聚异麦芽糖0.80%(W/V)、低聚木糖0.40%(W/V),在最优条件下合生元酸马乳中乳酸菌活菌数可达到(5.97±0.16)×10~8 CFU/m L,比优化前提高了70.57%。

Abstract: In order to study the effects of different oligosaccharides on the growth of lactic acid bacteria in synbiotics koumiss,three oligosaccharides for promoting the growth of lactic acid bacteria were screened via single factor experiment,and the optimal additions of three substrates were confirmed by central composite design and response surface analysis. The results indicated that the optimal additions of three oligosaccharides were 1.30%(W/V) of galacto-oligosaccharides,0.80%(W/V) of isomaltooligosaccharide and 0.40%(W/V) of xylooligosaccharides. Under the optimal conditions,the viable counts of lactic acid bacteria could reach up to(5.97±0.16)×10~8 CFU/m L,which revealed an increase by 70.57%.