Abstract: In order to investigate the α- glucosidase inhibitory activity of Camellia bee pollen flavonoids,10 kinds of flavonoid extracts of Camellia bee pollen prepared by different methods were chosen for flavonoids content and α- glucosidase inhibitory activity tset. The flavonoids content was increased markedly after dispose by AB- 8macroporous resins,the flavonoids content( 180.17 mg RE / g) and α- glucosidase inhibitory( 82.67%,5 mg / m L) of fractions eluted by 50% ethanol from AB-8 resin were all higher than other fractions.The results of correlation study showed that the correlation between total flavonoids content and α- glucosidase inhibitory activity in Camellia bee pollen was significantly positive( p < 0.01) and the correlation coefficient was 0.867. Enzyme dynamic analysis showed that the half- inhibitory concentration( IC₅₀) of flavonoid extract of Camellia bee pollen on α- glucosidase was determined to be 1.27 mg / m L.The inhibition was detected to belong to noncompetitive reversible inhibition with a inhibition constant( Ki) of 1.17 mg / m L.These results provided a theoretical reference for further comprehensive utilization of flavonoids and α- glucosidase inhibitors from bee pollen.