蚕蛹虫草片剂对体液免疫和细胞免疫功能的影响

张岚; 王丹; 聂晶石

doi:10.13386/j.issn1002-0306.2016.05.062

蚕蛹虫草片剂对体液免疫和细胞免疫功能的影响

Effect of Cordyceps militaris on humoral immunity and cellular immunity

摘要

摘要: 本文研究了蛹虫草片剂对小鼠体液免疫和细胞免疫功能影响。将雄性ICR小鼠随机分为I、II、III三个大组,每大组随机分为阴性对照组、蛹虫草低(0.167 g/kg·bw)、中(0.333 g/kg·bw)、高(0.999 g/kg·bw)剂量组,每组10只。每天灌胃一次,连续30 d。分别进行淋巴细胞转化实验、迟发型变态反应实验、并测定抗体生成细胞及血清溶血素。结果表明,与阴性对照组相比,蛹虫草片剂对Con A诱导小鼠的体重,脾脏/体重比值,胸腺/体重比值无显著性影响(p>0.05)。蛹虫草中、高剂量组对Con A诱导小鼠细胞免疫调节有显著性影响(p<0.05);蚕蛹虫草对小鼠体液免疫功能也具有一定调节作用,蛹虫草低、中、高剂量组对抗体生成细胞作用均有显著性影响(p<0.05),且中、高剂量组对HC50值影响有显著性影响(p<0.05)。说明一定剂量的蛹虫草片具有调节机体免疫能力,也为蛹虫草片剂对小鼠免疫调节机制研究奠定基础。

Abstract: In order to study effect of Cordyceps militaris on humoral immunity and cellular immunity functions of mice.ICR male mice were assigned randomly to I,II,III groups.Every group was assigned to the following groups:negative control,low( 0.167 g / kg·bw),medium( 0.333 g / kg·bw),high( 0.999 g / kg·bw) dosage CM for 30 days by intragastric infusion. Then spleen lymphocyte proliferation,delayed type hypersensitivity,quantity of antibody forming cells,and hemolytic value( HC₅₀) were detected. The results showed that Cordyceps militaris had no significant effect on body weight increase,ratio of spleen weight to body weight,ratio of thymus weight to body weight( p > 0.05). Medium and high dose groups had significant effect on cellular immunity( p < 0.05); and had significant effect on humoral immunity,all groups had significant effect on quantity of antibody forming cells,and medium and high dose groups had significant effects on HC₅₀( p < 0.05). So it was concluded that Cordyceps militaris can strengthen immunity functions of mice,and lay the foundation for mechanism research of immune regulation.