Abstract: The contents of human milk total protein,whey protein and casein were determined using Lowry,BCA and Bradford,respectively,and the results were compared with the Kjeldahl. The results showed that both Lowry and Bradford methods were suitable for determination of total protein content,while the whey protein content determined using Lowry method was closer to Kjeldahl. Lowry,BCA and Bradford methods were applicable for determination of the content of casein. Adopt the above- mentioned methods,the contents of human milk total protein,whey protein and casein can be rapidly determined within 1.5 h using only 4 m L human milk. These methods were suitable for the investigation of a large number of maternal and child nutritional status so that it provided the scientific basis for the development of human milk simulated infant formula and infant growth promotion.