响应面法优化怀菊水溶性总多酚的超声提取工艺

Optimization of ultrasonic- assisted extraction of total polyphenols from huai chrysanthemum by response surface analysis

摘要: 对怀菊中水溶性总多酚的超声辅助提取工艺进行了优化。以水作提取剂,在单因素实验的基础上选择超声功率、超声时间、液料比3个因素为自变量,以怀菊中总多酚的得率为响应值,进行中心组合设计(CCD),采用响应面法优化了怀菊总多酚的提取工艺。结果表明,超声辅助提取怀菊中总多酚的最佳工艺条件为:超声功率225 W,超声处理时间为40 min,液料比23.5∶1(m L/g)。在此工艺条件下,总多酚的得率高达76.53 mg/g。该实验结果能够为怀菊中水溶性多酚提取工艺的进一步放大提供科学依据和理论参考。

Abstract: Response surface methodology was applied to optimize ultrasonic- assisted extraction process of total polyphenols from huai chrysanthemum based on single experiments.Ultrasonic power,extraction times and ratio of solid and liquid were selected for central composite design( CCD). The response surface method was used to optimize the huai chrysanthemum the extraction process of total polyphenols.The results indicated that the optimal process conditions were as followings: ultrasonic power was 225 W,extraction time was 40 min,the ratio of liquid and solid was 23.5∶ 1( m L / g).Under these optimized conditions,the yield of total polyphenols was up to 76.53 mg /g.These researches provided some useful and valid reference for the further study of huai chrysanthemum.