Abstract: This article had a preliminary discussion on the best scheme of the extraction of the seed oil in the fruit of acanthopanax,and carried out the gas chromatographic analysis on the components of the seed oil and volatile oil in the fruit of acanthopanax. The best extraction conditions of the seed oil in acanthopanax were determined as follows:feed liquid proportion 1∶15,ultrasonic power 150 W,ultrasonic time 20 min. The highest extraction rate of the seed oil under such extraction conditions was 14.83%. The gas chromatographic analysis is carried out on the acquired seed oil in acanthopanax to identify its 13 components,and the key components included 18. 81 % of oleic acid in content, 14. 70 % of α- bisabolol, 14. 67 % of Trans squalene, 14. 04 % of nonacosane. The gas chromatographic analysis was carried out on the volatile oil in acanthopanax to identify its 16 components,and the key components include 20.52% of α-bisabolol in content,18.63% of caryophyllene,7.93% of cyclohexene.