Abstract: The response surface analysis method was used to study the Ginkgo protein extraction process on the basis of single factor experiment with Ginkgo for materials,the effect of Tris-HCl concentration,p H,solidliquid rate,extraction time on the protein extraction yield was inspected. The research results showed that the optimal conditions for ginkgo protein extraction:0.16 mol/L,p H8.7,1∶26(g/m L),and 5 h. Under this condition,the yield of Ginkgo protein was 82.76%,compared to the theoretical value,the relative error of 0.30%. Optimized regression equation derived some practical significance.