Abstract: Mercury is identified as one of the most toxic element and its toxicity is closely related to the speciation of mercury.Aquatic product is the main source of mercury ingested by human being.Large amount of consumption of aquatic products polluted by mercury may bring serious threat to human health.Current knowledge on the source and species of mercury,the hygienic standard of mercury in domestic and overseas aquatic products,sample pretreatment,the separation and detection,and validation of the method during the analysis of mercury speciation were summarized in this review,which provided technological references for the research of quality and safety of aquatic products.