Abstract: An analytical method has been established to detect 5 intense sweeteners( Acesulfame- k,Sodium saccharin,Cyclamate,Sucralose,and Aspartame) simultaneously in beverages by reverse phase high performance liquid chromatography( RP- HPLC). The separation process was carried out on a HALO column with methanol /formic acid—triethylamine buffer solution( p H = 4.5) as mobile phase with gradient elution. The quanlification and quantitation were accomplished by the evaporative light scattering detector. The 5 sweeteners were baseline separated in 4.5 minutes.The linear range was from 5 μg / m L to 500 μg / m L,with correlation coefficient greater than0.997.The LOD was 2 μg / m L and LOQ was 5 μg / m L.An average recovery was between 83.0% to 106.7% and the RSD was 0.84% ~ 3.37%. The results indicated that the method was simple,quick,serviceable and applicable to detection of sweeteners in beverage,it was also suitable for the detection of no ultraviolet absorption of sweeteners.