响应面法优化牛骨制备骨多肽工艺的研究

Study on enzymatic process of bovine bone for the peptides by response surface optimization

摘要: 以牛骨为原料,利用蛋白酶对牛骨水解进行单因素实验,在单因素基础上,根据中心组合实验设计原理运用响应面法,以水解度和多肽得率为响应值,得到最佳酶解条件为:酶解时间3.45 h,加酶量10500 U/g,底物浓度12%,温度45℃,p H7.5,此参数下水解度(DH)为14.29%,多肽得率69.76%,接近响应面预测值。结果表明,酶水解牛骨可以有效分解骨蛋白产生短链肽类,对牛骨蛋白利用以及开发活性肽有十分重要意义,为牛骨资源的精深加工提供实验依据。

Abstract: Bovine bone as the raw material,the enzymatic process for bone peptide was studied.Then on the basis of single- factor experiments,response surface analysis was adopted according to Box- Behnken center- united experimental design principle with hydrolysis degree and the rate of peptides as the responses. The optimum enzymolysis condition was obtained as follows: substrate concentration was 12%,hydrolysis p H was 7.5,enzyme concentration was 10500 U / g,hydrolysis temperature was 45 ℃,and hydrolysis time was 3.45 h,in this condition the rate of peptides and hydrolysis degree are 69.76% and 14.29%.The predicted values for optimization process conditions were in good agreement with experimental data.The results showed that enzymatic hydrolysis of bovine bone can produce peptides and provide experimental basis for the intensive processing of bone resources.