Abstract: A ethanol- water solution was used as mobile phase,and the HPD- 100 macroporous resin as the stationary phase to separate the vinyl part from ginger oleoresin extracted by supercritical carbon dioxide,and HPLC was used for detecting vinyls tracing.Different developing solvents of vinyls on argentated silica gel G precoated plate were compared,V_hexane∶ V₍ethylacetate)= 95 ∶ 5 was determined as the mobile phase to sepatateβ- sesquiphellandrene. The GC / MS test results indicated that HPD- 100 macroporous resin and silver nitrate modified silica gel were separately used as the stationary phase which could effectively separateβ- sesquiphellandrene from ginger oleoresin with 88.73% area percentage and 84.05% recovery. Through further HPLC analysis,β- sesquiphellandrene was found its existence of UV absorption at 232 nm.