桔梗糖蛋白超临界辅助提取工艺及对α-葡萄糖苷酶抑制作用的研究

赵胜男; 郑晓凤; 崔琳; 廉亚楠; 林雪燕; 果婷婷; 谷娜; 高金波

doi:10.13386/j.issn1002-0306.2016.03.031

桔梗糖蛋白超临界辅助提取工艺及对α-葡萄糖苷酶抑制作用的研究

Study on the optimum conditions of the extraction and inhibition ofα-glucosidase of glycoprotein from Platycodon grandiflorum with supercritical CO2 and ultrasonic

摘要

摘要: 确定超临界CO₂萃取辅助超声波提取桔梗糖蛋白的最佳工艺与其对α-葡萄糖苷酶抑制作用。以乙醇为夹带剂,采用超临界CO₂萃取技术辅助超声提取桔梗中的糖蛋白,以得率及其多糖含量为检测指标,对提取工艺进行评价;以PNPG为底物测定对α-葡萄糖苷酶抑制作用。结果表明:超临界CO₂萃取的最佳工艺为:温度45℃,压力25 MPa,乙醇浓度为70%,萃取时间为1.5 h;其平均得率和糖含量分别为27.83%和62.68%,糖蛋白和阿卡波糖对α-葡萄糖苷酶的半数抑制浓度IC₅₀值分别为0.276 mg/m L和0.321 mg/m L。结果表明:超临界CO₂萃取技术结合超声提取桔梗糖蛋白的方法可行,步骤简单,无污染,桔梗糖蛋白对α-葡萄糖苷酶有较强的抑制作用。

Abstract: To determine the optimum conditions of the extraction and explore the inhibition of α- glucosidase of glycoprotein from Platycodon grandiflorum with supercritical CO₂ and ultrasonic.Ethyl alcohol was used as entrainer to extract the glycoprotein from Platycodon grandiflorum with supercritical CO₂ and ultrasonic- assisted extraction.Extraction yield and the content of polysaccharide were as detection index to get the optimum conditions.When the extraction temperature was 45 ℃,extraction pressure was 25 MPa,entrainer was 70% ethyl ethanol and extraction time was 1.5 h,the average values of yield and content of the polysaccharide were 27.83% and 62.68%.The median inhibitory concentration on α- glycosidase activity of glycoprotein and Acarbose IC₅₀ were 0.276 mg / m L and0.321 mg / m L. The results showed that supercritical CO₂ combined with ultrasonic extraction of glycoprotein from Platycodon grandiflorum was feasible,simple and pollution- free.Platycodon glycoprotein had stronger inhibitory effect on α- glycosidase。