新疆野生樱桃李枝、叶不同萃取部位抗氧化活性研究
Study on antioxidant activity of different parts partitioned from Prunus divaricata Ldb branches and leaves in Xinjiang
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摘要: 采用系统溶剂萃取野生樱桃李枝、叶甲醇提取物,分别得到其石油醚相、乙酸乙酯相、正丁醇相,采用分光光度法测定其总酚含量,并通过清除DPPH自由基能力、还原能力、总抗氧化能力等化学模型对各部位的抗氧化活性进行测定。结果表明,野生樱桃李枝、叶提取物抗氧化活性与其总酚含量呈正相关性,提示酚类化合物为影响其抗氧化活性的主要因素。在三种抗氧化模型下,枝、叶各萃取相均表现一定的抗氧化活性,且具有相似的抗氧化活性规律:乙酸乙酯相>正丁醇相>石油醚相,说明乙酸乙酯和正丁醇可以有效地富集野生樱桃李枝、叶抗氧化成分,其中树枝乙酸乙酯相抗氧化活性著高于BHA。说明野生樱桃李枝、叶提取物具有作为天然抗氧化剂的开发价值。Abstract: The petroleum extract,ethyl acetate extract and n- butanol extract of Prunus divaricata Ldb branches and leaves were obtained by systematic solvent extraction,and their antioxidant activities were evaluated by scavenging activity of DPPH radicals,reducing power and total antioxidant activity.The total phenolic content in the obtained extracts were measured.The results showed that the antioxidant activities of the extracts were positively correlated to their total phenolic content,it showed that total phenolic content were main factors for the antioxidant activities.Each part of extracts had antioxidant activities and similar tendency,the ethyl acetate extract displayed the strongest activity,followed by n- butanol extract and the petroleum extract.This indicated that the ethyl acetate extract and n- butanol extract were rich in antioxidant components.The ethyl acetate extract of branches had the strongest activity,remarkably higher than that of BHA. The total phenolic content from Prunus divaricata Ldb branches and leaves could be produced as natural antioxidants.
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