香蕉茎秆汁液抑制晚期糖基化终末产物活性评价

商文婷; 盛占武; 谷满屯; 郑丽丽; 艾斌凌; 杨劲松

doi:10.13386/j.issn1002-0306.2016.03.001

香蕉茎秆汁液抑制晚期糖基化终末产物活性评价

Inhibition to advanced glycation end- products and activity evaluation of sap of banana stem

摘要

摘要: 为研究香蕉茎秆汁液抑制晚期糖基化终末产物（AGEs）活性,选用α-葡萄糖苷酶和α-淀粉酶抑制模型研究其对碳水化合物代谢关键酶的抑制作用,并采用Lineweaver-Burk双倒数法研究其动力学性质。同时对AGEs的抑制和清除ABTS⁺·的能力也进行了评价。结果表明,香蕉茎秆汁液对α-葡萄糖苷酶的半抑制浓度(IC₅₀)为1.51 mg/m L,对α-葡萄糖苷酶的抑制类型为混合型抑制。香蕉茎秆汁液对α-淀粉酶的半抑制浓度(IC₅₀)是8.64 mg/m L,抑制类型为不可逆抑制。通过实验研究,香蕉茎秆汁液具有一定清除ABTS⁺·的能力,对牛血清白蛋白果糖模型AGEs也具有一定的抑制作用。香蕉茎秆汁液可抑制体外AGEs的生成。

Abstract: To study the inhibition to advanced glycation end- products( AGEs) activity by sap of banana stem,the inhibition of key enzymes in carbohydrate metabolism was evaluated by using α- glucosidase and α- amylase inhibition model.Inhibitory kinetic parameters were determined by Lineweaver- Burk plot.Meanwhile,the abilities of inhibiting AGEs and removing ABTS⁺· were also evaluated.The results showed that the median inhibitory concentration( IC₅₀) of sap of banana stem on α-glucosidase was 1.51 mg /m L and the type of inhibition was mixcompetitive inhibition. The median inhibitory concentration( IC₅₀) of sap of banana stem on α- amylase was8.64 mg / m L and the type of inhibition was irreversible inhibition. Through experiment,sap of banana stem had a scavenging activity on removing ABTS⁺·also had a certain capacity of inhibiting AGEs.The sap of banana stem had a certain inhibitory activity against AGEs.