响应面优化复合酶法提取竹笋膳食纤维工艺

Optimization of complex enzyme extraction condition of dietary fiber from bamboo shoots using response surface analysis

摘要: 以竹笋为原料,在酶解时间、酶解温度、复合酶质量分数、复合酶质量比值4个单因素实验的基础上,通过响应面法对竹笋膳食纤维提取工艺条件进行优化。结果表明:最佳提取工艺条件为酶解时间95 min、酶解温度56℃、复合酶质量分数0.52%、复合酶质量比值为蛋白酶∶纤维素酶=3∶1,在此条件下竹笋膳食纤维提取率最大,为53.21%。

Abstract: Bamboo shoots was used as raw material to optimize the complex enzyme extraction condition of dietary fiber through response surface methodology on the basis of four single factor : time, temperature,complex enzyme adding dose and the quality of compound enzyme ratio.The results showed that the optimum extraction conditions as followings:time was 95 minutes,temperature was 56 ℃,complex enzyme adding dose was 0.52%,the mass ratio of protease to cellulase was 3 ∶1. Under these conditions,the experimental yield of bamboo shoot dietary fiber was 53.21%,which was the largest.